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KMID : 0613820060160040691
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Journal of Life Science
2006 Volume.16 No. 4 p.691 ~ p.698
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Regulation of Phospholipase D by CoCl₂ in Human Glioblastoma Cells
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Lee Seung-Hoon
Min Gye-Sik
Min Do-Sik
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Abstract
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Phospholipase D (PLD) is known to play an important role in a variety of cells. However, little is known about CoCl₂-mediated PLD signaling. In this study we demonstrated for the first time that CoCl₂ stimulates PLD activity and increases expression of cyclooxygenase-2 (COX-2), which is known to mediate inflammatory reaction. CoCl₂-induced PLD activity was assessed by measuring the formation of [3H] phosphatidylbutanol (PtdBut), the product of PLD-mediated transphosphatidylation, in the presence of 1-butanol. To study mechanism of PLD signaling induced by CoCl₂, U87 human glioblastoma cells were stimulated by CoCl₂ and regulators of PLD activity induced by CoCl₂ were investigated using several inhibitors of signaling proteins. Moreover, PLD activation by CoCl₂ increased not only expression of COX-2 protein but also COX-2 promoter activity. In summary, these results suggest that CoCl₂ increases expression of COX-2 protein via PLD in human U87 glioblastoma cells.
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KEYWORD
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CoCl₂, phospholipase D, glioma, COX-2
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